Poster #062
Clone a fluorescent reporter from GFP-marker to mCherry-marker for reading
Mentors: PI: Jamieson Catriona, M.D., Ph.D. Lab Mentors: Jessica Pham, Kendale Wirtjes
Our project aimed to replace a green fluorescent marker (GFP) with a red marker (mCherry) to read out ADAR1-specific A-to-I editing activity. We focused on the question: Can the gene of interest be removed using a linearized vector and primers through PCR? After cloning an ADAR1 Vector and using plasmid construction, the mCherry was inserted to replace the present gene (GFP), allowing the promoter to activate the new fluorescent reporter. The samples were run alongside a ladder on an agarose gel to compare the base pairs, confirming the success of the cloning assay. The plasmid map and primer design have been completed, and have correct alignment. The gel electrophoresis to confirm the size and DNA purification are in progress. Combining our research with other tools enables scientists to better understand RNA editing activity controlled by the ADAR1 enzyme and how certain levels may be linked to diseases.