Poster #022
Validation of Fibroblast-Derived iPSCs via Tri-Lineage Differentiation
Mentors: Dr. Junjing Jia, PhD; PI: Dr. Junjing Jia, PhD
Induced pluripotent stem cells (iPSCs) offer a patient-specific platform for disease modeling and regenerative medicine. In this study, dermal fibroblasts collected through patient skin punch biopsy samples were isolated, cultured, and reprogrammed into iPSCs using established methods. Pluripotency was confirmed through immunocytochemistry, demonstrating strong expression of key stem cell markers including SSEA-4, Tra-1-60, OCT4, and SOX2, with DAPI staining used to visualize cell nuclei. The resulting iPSC colonies exhibited expected structure and clear fluorescence in both surface and nuclear regions. To evaluate their ability to develop into different cell types, tri-lineage differentiation was performed using the STEMdiff Trilineage Differentiation Kit and analyzed using fluorescent antibody staining. Imaging conducted via Nikon Ti-U fluorescence microscope displayed the presence of early germ layer markers and confirmed successful differentiation into ectoderm (PAX6, Nestin), mesoderm (Brachyury, NCAM), and endoderm (SOX17, FOXA2) layers. These iPSC lines will support future CNKSR2-mutation research and broader patient-specific disease modeling.